Hotspot

psbB

LSC · 71973-73499 · 1527 bp

Back to Gynochthodes

Pi 0.00131

MarkerSeek score 49.50

Primer available yes

Label psbB

Feature Metadata

label_name: psbB
feature_type: gene
region: LSC
length_bp: 1527
variable_sites: 3
pi: 0.00131
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbB_p1	ATCCACTGAACAAGGGAGGT	GGCGTAAAGATACCAAAAGGG	50.0	47.6	58.3	57.0	1657 bp	1.0	61.7
psbB_p2	TCCACTGAACAAGGGAGGTA	GGCGTAAAGATACCAAAAGGG	50.0	47.6	57.6	57.0	1656 bp	1.0	60.5
psbB_p3	CCACTGAACAAGGGAGGTAC	GGCGTAAAGATACCAAAAGGG	55.0	47.6	57.5	57.0	1655 bp	1.0	60.0
psbB_p4	ACCCTGTTCGGAAAGAATCC	GGCGTAAAGATACCAAAAGGG	50.0	47.6	57.5	57.0	1673 bp	1.0	59.8
psbB_p5	ATCCACTGAACAAGGGAGGT	AGGCGTAAAGATACCAAAAGGG	50.0	45.5	58.3	58.4	1658 bp	1.0	59.1