Hotspot

ndhA

SSC · 118572-120764 · 2193 bp

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Pi 0.00365

MarkerSeek score 59.70

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2193
variable_sites: 12
pi: 0.00365
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	TGTTTGAGACAAGTCGTGAA	GTCCACCGGGGTTTATCAAT	40.0	50.0	55.2	57.6	2352-2359 bp	1.0	54.6
ndhA_p2	TTGTTTGAGACAAGTCGTGA	GTCCACCGGGGTTTATCAAT	40.0	50.0	55.2	57.6	2353-2360 bp	1.0	54.6
ndhA_p3	TTGTTTGAGACAAGTCGTGAA	GTCCACCGGGGTTTATCAAT	38.1	50.0	56.0	57.6	2353-2360 bp	1.0	53.0
ndhA_p4	TGAGACAAGTCGTGAATACAGA	GTCCACCGGGGTTTATCAAT	40.9	50.0	56.9	57.6	2348-2355 bp	1.0	52.1
ndhA_p5	TGTTTGAGACAAGTCGTGAAT	GTCCACCGGGGTTTATCAAT	38.1	50.0	55.8	57.6	2352-2359 bp	1.0	51.2