Hotspot

accD

LSC · 56786-58360 · 1575 bp

Back to Gynochthodes

Pi 0.00381

MarkerSeek score 61.00

Primer available yes

Label accD

Feature Metadata

label_name: accD
feature_type: gene
region: LSC
length_bp: 1575
variable_sites: 9
pi: 0.00381
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD_p1	TGAGTTTCCCTGAAGCAAGG	ACGAAACCAACAAAAATTCCCT	50.0	36.4	57.7	57.3	1727 bp	1.0	56.2
accD_p2	TGAGTTTCCCTGAAGCAAGG	ACGAAACCAACAAAAATTCCCTT	50.0	34.8	57.7	57.9	1727 bp	1.0	53.5
accD_p3	TGAGTTTCCCTGAAGCAAGG	ACGAAACCAACAAAAATTCCC	50.0	38.1	57.7	55.8	1727 bp	1.0	52.5
accD_p4	TGAGTTTCCCTGAAGCAAGG	TCCCTTCTTCCCTTCTTAGT	50.0	45.0	57.7	54.7	1710 bp	1.0	52.0
accD_p5	GGCTTTTCTACCCATCCTGT	ACGAAACCAACAAAAATTCCCT	50.0	36.4	57.2	57.3	1709 bp	1.0	52.0