Hotspot

rpl20-rps12

LSC · 68454-69211 · 758 bp

Back to Gynochthodes

Pi 0.00176

MarkerSeek score 59.90

Primer available yes

Label rpl20-rps12

Feature Metadata

label_name: rpl20-rps12
feature_type: igs
region: LSC
length_bp: 758
variable_sites: 2
pi: 0.00176
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl20-rps12_p1	GTAAGTCTTGAATGGGCCCC	GAGGAACATGTACTCGGGTG	55.0	55.0	58.2	57.7	876 bp	1.0	75.9
rpl20-rps12_p2	AGCTTGACGCGAATAAACGA	GAGGAACATGTACTCGGGTG	45.0	55.0	58.3	57.7	851 bp	1.0	75.5
rpl20-rps12_p3	GTAAGTCTTGAATGGGCCCC	AGGAACATGTACTCGGGTGT	55.0	50.0	58.2	58.4	875 bp	1.0	75.4
rpl20-rps12_p4	TTTGTTCTACGTCTCCGAGC	GAGGAACATGTACTCGGGTG	50.0	55.0	57.7	57.7	828 bp	1.0	75.1
rpl20-rps12_p5	AGCTTGACGCGAATAAACGA	AGGAACATGTACTCGGGTGT	45.0	50.0	58.3	58.4	850 bp	1.0	75.0