Hotspot

petA-psbJ

LSC · 62674-63590 · 917 bp

Back to Gynochthodes

Pi 0.0124

MarkerSeek score 66.60

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 917
variable_sites: 17
pi: 0.0124
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	GGCCTTTTGTTCTTCTTCGC	ATTCCGGATTGGGTTCATCC	50.0	50.0	57.9	57.6	1045 bp	1.0	78.0
petA-psbJ_p2	TGTCCAAGGCCTTTTGTTCT	ATTCCGGATTGGGTTCATCC	45.0	50.0	57.5	57.6	1052 bp	1.0	74.8
petA-psbJ_p3	GTGTCCAAGGCCTTTTGTTC	ATTCCGGATTGGGTTCATCC	50.0	50.0	57.5	57.6	1053 bp	1.0	74.3
petA-psbJ_p4	GCCTTTTGTTCTTCTTCGCA	ATTCCGGATTGGGTTCATCC	45.0	50.0	57.2	57.6	1044 bp	1.0	72.2
petA-psbJ_p5	TCGAGAAGGTTCAATTGGCT	ATTCCGGATTGGGTTCATCC	45.0	50.0	57.1	57.6	978 bp	1.0	71.6