Hotspot

ycf4-cemA

LSC · 60161-60780 · 620 bp

Back to Gynochthodes

Pi 0.00215

MarkerSeek score 60.10

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 620
variable_sites: 2
pi: 0.00215
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	AACAAAAGGCTGCCGAATTG	GAGAAAGACACCAGGGCAAA	45.0	50.0	57.8	58.0	749 bp	1.0	78.8
ycf4-cemA_p2	AACAAAAGGCTGCCGAATTG	TTTAACGGGGGAGTGAATGC	45.0	50.0	57.8	58.2	711 bp	1.0	77.6
ycf4-cemA_p3	AACAAAAGGCTGCCGAATTG	GGGGGAGTGAATGCTTTCTT	45.0	50.0	57.8	57.8	705 bp	1.0	77.2
ycf4-cemA_p4	GAACAAAAGGCTGCCGAATT	GAGAAAGACACCAGGGCAAA	45.0	50.0	57.6	58.0	750 bp	1.0	76.6
ycf4-cemA_p5	ACATTGAACAAAAGGCTGCC	GAGAAAGACACCAGGGCAAA	45.0	50.0	57.5	58.0	755 bp	1.0	75.8