Hotspot

accD-psaI

LSC · 58361-59090 · 730 bp

Back to Gynochthodes

Pi 0.00824

MarkerSeek score 57.40

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 730
variable_sites: 9
pi: 0.00824
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	GTTCCATGCTTTTCTTCCTTTGA	CCATTGCAATTGCCGGAAAT	39.1	45.0	57.8	58.0	757-846 bp	1.0	59.1
accD-psaI_p2	GTTCCATGCTTTTCTTCCTTTGA	AAGAAGCCATTGCAATTGCC	39.1	45.0	57.8	57.9	763-852 bp	1.0	58.4
accD-psaI_p3	TCCATGCTTTTCTTCCTTTGA	CCATTGCAATTGCCGGAAAT	38.1	45.0	55.7	58.0	755-844 bp	1.0	58.1
accD-psaI_p4	TCCATGCTTTTCTTCCTTTGA	AAGAAGCCATTGCAATTGCC	38.1	45.0	55.7	57.9	761-850 bp	1.0	57.5
accD-psaI_p5	TGCTTTTCTTCCTTTGAATCCA	CCATTGCAATTGCCGGAAAT	36.4	45.0	56.4	58.0	751-840 bp	1.0	56.2