Hotspot

trnF-GAA-ndhJ

LSC · 48515-49223 · 709 bp

Back to Gynochthodes

Pi 0.0019

MarkerSeek score 61.00

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 709
variable_sites: 2
pi: 0.0019
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 61.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	GGGATAGCTCAGCTGGTAGA	ATCCACGCCTGAAACGTATC	55.0	50.0	58.0	58.1	875-885 bp	1.0	80.4
trnF-GAA-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	ATCCACGCCTGAAACGTATC	50.0	50.0	58.1	58.1	839-849 bp	1.0	80.0
trnF-GAA-ndhJ_p3	GGGATAGCTCAGCTGGTAGA	GCCGGAAAGTTGGATAGGTT	55.0	50.0	58.0	57.9	850-860 bp	1.0	79.9
trnF-GAA-ndhJ_p4	CCTCGTGTCACCAGTTCAAA	GCCGGAAAGTTGGATAGGTT	50.0	50.0	58.1	57.9	814-824 bp	1.0	79.6
trnF-GAA-ndhJ_p5	CTGGTAGAGCAGAGGACTGA	ATCCACGCCTGAAACGTATC	55.0	50.0	57.9	58.1	863-873 bp	1.0	79.3