Hotspot

psaA-ycf3

LSC · 42083-42990 · 908 bp

Back to Gynochthodes

Pi 0.0022

MarkerSeek score 60.20

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 908
variable_sites: 3
pi: 0.0022
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	ACGCGATCCCTATCTACCAA	TTGAAGATCACGAGGCGTTT	50.0	45.0	58.0	57.8	991-1008 bp	1.0	78.9
psaA-ycf3_p2	ACGCGATCCCTATCTACCAA	TTGGTTGAAGATCACGAGGC	50.0	50.0	58.0	58.2	995-1012 bp	1.0	78.6
psaA-ycf3_p3	CCTGGTTTAGCCCATTCCTC	TTGAAGATCACGAGGCGTTT	55.0	45.0	57.9	57.8	1024-1041 bp	1.0	78.5
psaA-ycf3_p4	CCTGGTTTAGCCCATTCCTC	TTGGTTGAAGATCACGAGGC	55.0	50.0	57.9	58.2	1028-1045 bp	1.0	78.2
psaA-ycf3_p5	ACGCGATCCCTATCTACCAA	TGAAGATCACGAGGCGTTTC	50.0	50.0	58.0	58.3	990-1007 bp	1.0	77.9