Hotspot

trnE-UUC-trnT-GGU

LSC · 30946-31714 · 769 bp

Back to Gynochthodes

Pi 0.00347

MarkerSeek score 61.90

Primer available yes

Label trnE-UUC-trnT-GGU

Feature Metadata

label_name: trnE-UUC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 769
variable_sites: 4
pi: 0.00347
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnT-GGU_p1	TCTTTCGTAGTACCCCCAGG	GAACCGATGACTTACGCCTT	55.0	50.0	58.1	58.0	901-933 bp	1.0	79.7
trnE-UUC-trnT-GGU_p2	TCTTTCGTAGTACCCCCAGG	TACCGCTGAGTTAAAAGGGC	55.0	50.0	58.1	57.9	866-898 bp	1.0	78.9
trnE-UUC-trnT-GGU_p3	GCTGCCTCCTTGAAAGAGAG	GAACCGATGACTTACGCCTT	55.0	50.0	58.3	58.0	866-898 bp	1.0	78.8
trnE-UUC-trnT-GGU_p4	TGTCCTGAACCACTAGACGA	GAACCGATGACTTACGCCTT	50.0	50.0	57.7	58.0	845-877 bp	1.0	78.6
trnE-UUC-trnT-GGU_p5	TCTTTCGTAGTACCCCCAGG	CCGATGACTTACGCCTTACC	55.0	55.0	58.1	58.2	898-930 bp	1.0	78.5