Hotspot

trnS-GCU-trnG-UCC

LSC · 8523-9733 · 1211 bp

Back to Gynochthodes

Pi 0.00276

MarkerSeek score 62.30

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 1211
variable_sites: 5
pi: 0.00276
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	CAATCCGACGCTTTAGTCCA	CATCGTTAGCTTGGAAGGCT	50.0	50.0	58.0	58.0	1343-1361 bp	1.0	81.1
trnS-GCU-trnG-UCC_p2	AATCCGACGCTTTAGTCCAC	CATCGTTAGCTTGGAAGGCT	50.0	50.0	58.0	58.0	1342-1360 bp	1.0	81.1
trnS-GCU-trnG-UCC_p3	CAATCCGACGCTTTAGTCCA	TCAAAACCGAACCCGAAACT	50.0	45.0	58.0	58.0	1273-1291 bp	1.0	81.1
trnS-GCU-trnG-UCC_p4	AATCCGACGCTTTAGTCCAC	TCAAAACCGAACCCGAAACT	50.0	45.0	58.0	58.0	1272-1290 bp	1.0	81.1
trnS-GCU-trnG-UCC_p5	CAATCCGACGCTTTAGTCCA	CGTTAGCTTGGAAGGCTAGG	50.0	55.0	58.0	58.1	1340-1358 bp	1.0	80.9