Hotspot

rpoB

LSC · 25030-28242 · 3213 bp

Back to Gymnotheca

Pi 0.00156

MarkerSeek score 42.20

Primer available yes

Label rpoB

Feature Metadata

label_name: rpoB
feature_type: gene
region: LSC
length_bp: 3213
variable_sites: 5
pi: 0.00156
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 42.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB_p1	AACTCGATTTTCGCCCATGA	TCAATTCGGATTGGCTCTGG	45.0	50.0	57.9	58.0	215 bp	1.0	79.5
rpoB_p2	ACTCGATTTTCGCCCATGAA	TCAATTCGGATTGGCTCTGG	45.0	50.0	57.9	58.0	214 bp	1.0	79.5
rpoB_p3	TCGATTTTCGCCCATGAACT	TCAATTCGGATTGGCTCTGG	45.0	50.0	57.9	58.0	212 bp	1.0	79.5
rpoB_p4	ACTCGATTTTCGCCCATGAA	ATTCGGATTGGCTCTGGTTC	45.0	50.0	57.9	58.0	211 bp	1.0	79.5
rpoB_p5	TCGATTTTCGCCCATGAACT	ATTCGGATTGGCTCTGGTTC	45.0	50.0	57.9	58.0	209 bp	1.0	79.5