Hotspot

psbC

LSC · 36556-37977 · 1422 bp

Back to Gymnotheca

Pi 0.00141

MarkerSeek score 59.60

Primer available yes

Label psbC

Feature Metadata

label_name: psbC
feature_type: gene
region: LSC
length_bp: 1422
variable_sites: 2
pi: 0.00141
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbC_p1	GCAGCGGAAGATCCTGAATT	ACTTCATGCATTCGATCCCC	50.0	50.0	58.3	58.0	1540 bp	1.0	77.3
psbC_p2	GCAGCGGAAGATCCTGAATT	TTCATGCATTCGATCCCCTG	50.0	50.0	58.3	58.0	1538 bp	1.0	77.3
psbC_p3	AGGTATTCGTGCTTGGATGG	ACTTCATGCATTCGATCCCC	50.0	50.0	57.7	58.0	1484 bp	1.0	77.2
psbC_p4	AGGTATTCGTGCTTGGATGG	TTCATGCATTCGATCCCCTG	50.0	50.0	57.7	58.0	1482 bp	1.0	77.2
psbC_p5	AAATTCGTGCAGCGGAAGAT	ACTTCATGCATTCGATCCCC	45.0	50.0	58.5	58.0	1548 bp	1.0	75.5