Hotspot

ndhH

SSC · 128751-129932 · 1182 bp

Back to Gymnotheca

Pi 0.00169

MarkerSeek score 59.70

Primer available yes

Label ndhH

Feature Metadata

label_name: ndhH
feature_type: gene
region: SSC
length_bp: 1182
variable_sites: 2
pi: 0.00169
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhH_p1	GGGACAAGCATCCATATGAGT	TCGTTTTCGGTAATTCGTGGA	47.6	42.9	57.5	58.0	1321 bp	1.0	59.9
ndhH_p2	AGGGACAAGCATCCATATGAG	TCGTTTTCGGTAATTCGTGGA	47.6	42.9	57.2	58.0	1322 bp	1.0	57.7
ndhH_p3	GGGACAAGCATCCATATGAGT	CGTTTTCGGTAATTCGTGGA	47.6	45.0	57.5	56.5	1320 bp	1.0	56.0
ndhH_p4	GGGACAAGCATCCATATGAGT	TCGTTTTCGGTAATTCGTGG	47.6	45.0	57.5	56.5	1321 bp	1.0	56.0
ndhH_p5	AGGGACAAGCATCCATATGA	TCGTTTTCGGTAATTCGTGGA	45.0	42.9	56.0	58.0	1322 bp	1.0	55.7