Hotspot

ndhF

SSC · 116150-118369 · 2220 bp

Back to Gymnotheca

Pi 0.0027

MarkerSeek score 58.80

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2220
variable_sites: 6
pi: 0.0027
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	CCGATTGTGCCATGATGAGA	AGAGCTGGTGAATCAGAAACA	50.0	42.9	58.0	57.2	2366 bp	1.0	65.6
ndhF_p2	TCCGATTGTGCCATGATGAG	AGAGCTGGTGAATCAGAAACA	50.0	42.9	58.0	57.2	2367 bp	1.0	65.6
ndhF_p3	AAACGTATCCGATTGTGCCA	AGAGCTGGTGAATCAGAAACA	45.0	42.9	57.9	57.2	2374 bp	1.0	65.2
ndhF_p4	CGTATCCGATTGTGCCATGA	AGAGCTGGTGAATCAGAAACA	50.0	42.9	58.1	57.2	2371 bp	1.0	64.9
ndhF_p5	AACGTATCCGATTGTGCCAT	AGAGCTGGTGAATCAGAAACA	45.0	42.9	57.7	57.2	2373 bp	1.0	63.4