Hotspot

ccsA

SSC · 121084-122055 · 972 bp

Back to Gymnotheca

Pi 0.00412

MarkerSeek score 52.60

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 972
variable_sites: 4
pi: 0.00412
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	AAAGAAGGGGAGCACAATGG	TGGTTCTCAAAAACTCGCAA	50.0	40.0	58.1	56.1	1160 bp	1.0	64.6
ccsA_p2	AAAGAAGGGGAGCACAATGG	ATGGTTCTCAAAAACTCGCA	50.0	40.0	58.1	55.9	1161 bp	1.0	62.7
ccsA_p3	AAAGAAGGGGAGCACAATGG	TGGTTCTCAAAAACTCGCAAA	50.0	38.1	58.1	56.9	1160 bp	1.0	62.6
ccsA_p4	GAAGGGGAGCACAATGGATC	TGGTTCTCAAAAACTCGCAA	55.0	40.0	58.3	56.1	1157 bp	1.0	62.6
ccsA_p5	AAAGAAGGGGAGCACAATGG	ATGGTTCTCAAAAACTCGCAA	50.0	38.1	58.1	56.6	1161 bp	1.0	60.9