Hotspot

ccsA-ndhD

SSC · 122056-122300 · 245 bp

Back to Gymnotheca

Pi 0.0204

MarkerSeek score 60.10

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 245
variable_sites: 5
pi: 0.0204
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	GTGAATTCGGCAATTGTAGCT	CAGTTGACAAGGTAGAAGCGA	42.9	47.6	57.2	58.0	412-416 bp	1.0	58.6
ccsA-ndhD_p2	GTGAATTCGGCAATTGTAGCT	TCAGTTGACAAGGTAGAAGCG	42.9	47.6	57.2	58.0	413-417 bp	1.0	58.6
ccsA-ndhD_p3	GTGAATTCGGCAATTGTAGCTT	CAGTTGACAAGGTAGAAGCGA	40.9	47.6	57.9	58.0	412-416 bp	1.0	56.0
ccsA-ndhD_p4	GTGAATTCGGCAATTGTAGCTT	TCAGTTGACAAGGTAGAAGCG	40.9	47.6	57.9	58.0	413-417 bp	1.0	56.0
ccsA-ndhD_p5	CGGCAATTGTAGCTTCTATTGG	CAGTTGACAAGGTAGAAGCGA	45.5	47.6	57.8	58.0	405-409 bp	1.0	55.0