Hotspot

ndhF-rpl32

SSC · 118370-119582 · 1213 bp

Back to Gymnotheca

Pi 0.00578

MarkerSeek score 52.30

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 1213
variable_sites: 7
pi: 0.00578
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	TGCAGTCGGAACAAACAGAA	GTACGTTTCTTTGGAACCGC	45.0	50.0	58.0	57.7	1332 bp	1.0	77.8
ndhF-rpl32_p2	TTGCAGTCGGAACAAACAGA	GTACGTTTCTTTGGAACCGC	45.0	50.0	58.0	57.7	1333 bp	1.0	77.8
ndhF-rpl32_p3	ACGAAAGGTATGATCCACGC	GTACGTTTCTTTGGAACCGC	50.0	50.0	58.1	57.7	1277 bp	1.0	77.5
ndhF-rpl32_p4	CGAAAGGTATGATCCACGCA	GTACGTTTCTTTGGAACCGC	50.0	50.0	58.1	57.7	1276 bp	1.0	77.5
ndhF-rpl32_p5	GTTGCAGTCGGAACAAACAG	GTACGTTTCTTTGGAACCGC	50.0	50.0	57.9	57.7	1334 bp	1.0	77.2