Hotspot

petA-psbJ

LSC · 67373-68693 · 1321 bp

Back to Gymnotheca

Pi 0.00307

MarkerSeek score 61.90

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1321
variable_sites: 4
pi: 0.00307
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 61.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	GCATCTGTTACTCTGGCACA	CCTGTGATTGGTTTAGTGGGT	50.0	47.6	57.9	57.8	1452-1471 bp	1.0	71.2
petA-psbJ_p2	TTGGCATCTGTTACTCTGGC	CCTGTGATTGGTTTAGTGGGT	50.0	47.6	57.9	57.8	1455-1474 bp	1.0	71.1
petA-psbJ_p3	ACGTGTCCAAGGTTTGTTGT	CCTGTGATTGGTTTAGTGGGT	45.0	47.6	58.2	57.8	1480-1499 bp	1.0	70.6
petA-psbJ_p4	GCATCTGTTACTCTGGCACA	GAAGGATTCCTCTTTGGCTGA	50.0	47.6	57.9	57.6	1493-1512 bp	1.0	69.6
petA-psbJ_p5	TTGGCATCTGTTACTCTGGC	GAAGGATTCCTCTTTGGCTGA	50.0	47.6	57.9	57.6	1496-1515 bp	1.0	69.5