Hotspot

trnE-UUC-trnT-GGU

LSC · 33119-33806 · 688 bp

Back to Gymnotheca

Pi 0.00291

MarkerSeek score 60.40

Primer available yes

Label trnE-UUC-trnT-GGU

Feature Metadata

label_name: trnE-UUC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 688
variable_sites: 2
pi: 0.00291
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnT-GGU_p1	GCTGCCTCCTTGAAAGAGAG	GAACCGATGACTTACGCCTT	55.0	50.0	58.3	58.0	790 bp	1.0	78.0
trnE-UUC-trnT-GGU_p2	TTCGTAACACCTTACCCCCA	GAACCGATGACTTACGCCTT	50.0	50.0	58.3	58.0	827 bp	1.0	78.0
trnE-UUC-trnT-GGU_p3	TGACCACTAGACGATAGGGG	GAACCGATGACTTACGCCTT	55.0	50.0	57.6	58.0	764 bp	1.0	77.3
trnE-UUC-trnT-GGU_p4	GCTGCCTCCTTGAAAGAGAG	TACCGCTGAGTTAAAAGGGC	55.0	50.0	58.3	57.9	755 bp	1.0	77.3
trnE-UUC-trnT-GGU_p5	TTCGTAACACCTTACCCCCA	TACCGCTGAGTTAAAAGGGC	50.0	50.0	58.3	57.9	792 bp	1.0	77.2