Hotspot

rps2-rpoC2

LSC · 17713-17944 · 232 bp

Back to Gymnotheca

Pi 0.0129

MarkerSeek score 59.90

Primer available yes

Label rps2-rpoC2

Feature Metadata

label_name: rps2-rpoC2
feature_type: igs
region: LSC
length_bp: 232
variable_sites: 3
pi: 0.0129
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps2-rpoC2_p1	ATGACCGAAATGAACTCCCG	ATTCGAGGGGGAAATAAGCG	50.0	50.0	58.0	57.7	380 bp	1.0	78.4
rps2-rpoC2_p2	AATGAACTCCCGCTTCCATC	ATTCGAGGGGGAAATAAGCG	50.0	50.0	58.0	57.7	372 bp	1.0	78.2
rps2-rpoC2_p3	TGCAGAGATGTAAGGTCCCA	ATTCGAGGGGGAAATAAGCG	50.0	50.0	58.0	57.7	425 bp	1.0	78.1
rps2-rpoC2_p4	CCCGCTTCCATCATCTCTTC	ATTCGAGGGGGAAATAAGCG	55.0	50.0	58.1	57.7	364 bp	1.0	77.6
rps2-rpoC2_p5	GAAATGAACTCCCGCTTCCA	ATTCGAGGGGGAAATAAGCG	50.0	50.0	58.2	57.7	374 bp	1.0	77.2