Hotspot

trnS-GCU-trnG-UCC

LSC · 9032-9998 · 967 bp

Back to Gymnotheca

Pi 0.00311

MarkerSeek score 60.60

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 967
variable_sites: 3
pi: 0.00311
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	AGAAACCTCAAGGGATCCGA	ACGAATCACACTTTTACCACT	50.0	38.1	58.0	55.5	1113-1114 bp	1.0	51.6
trnS-GCU-trnG-UCC_p2	AGAAACCTCAAGGGATCCGA	AGAACGAATCACACTTTTACCAC	50.0	39.1	58.0	57.3	1116-1117 bp	1.0	50.6
trnS-GCU-trnG-UCC_p3	AGAAACCTCAAGGGATCCGA	GAACGAATCACACTTTTACCACT	50.0	39.1	58.0	57.3	1115-1116 bp	1.0	50.6
trnS-GCU-trnG-UCC_p4	GGAACGGAGAGAGAGGGATT	ACGAATCACACTTTTACCACT	55.0	38.1	58.2	55.5	1092-1093 bp	1.0	50.3
trnS-GCU-trnG-UCC_p5	AATCTGCCGCTTTAGTCCAC	ACGAATCACACTTTTACCACT	50.0	38.1	58.3	55.5	1034-1035 bp	1.0	49.9