Hotspot

trnT-UGU

LSC · 48198-48270 · 73 bp

Back to Gymnosporia

Pi 0.0137

MarkerSeek score 60.40

Primer available yes

Label trnT-UGU

Feature Metadata

label_name: trnT-UGU
feature_type: tRNA
region: LSC
length_bp: 73
variable_sites: 1
pi: 0.0137
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-UGU_p1	AGGGAAAGAGAAGCTTTTTCA	ACGAAGAGCTAAGATTCAACAGT	38.1	39.1	55.3	57.9	217 bp	1.0	40.4
trnT-UGU_p2	TGAAAAAGGGAAAGAGAAGCT	ACGAAGAGCTAAGATTCAACAGT	38.1	39.1	55.3	57.9	223 bp	1.0	40.4
trnT-UGU_p3	AAGGGAAAGAGAAGCTTTTTCA	ACGAAGAGCTAAGATTCAACAGT	36.4	39.1	56.1	57.9	218 bp	1.0	40.4
trnT-UGU_p4	AGGGAAAGAGAAGCTTTTTCAA	ACGAAGAGCTAAGATTCAACAGT	36.4	39.1	56.1	57.9	217 bp	1.0	40.4
trnT-UGU_p5	TGAAAAAGGGAAAGAGAAGCTT	ACGAAGAGCTAAGATTCAACAGT	36.4	39.1	56.1	57.9	223 bp	1.0	40.4