Hotspot

ndhD

SSC · 118610-120109 · 1500 bp

Back to Gymnosporia

Pi 0.00267

MarkerSeek score 60.30

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1500
variable_sites: 4
pi: 0.00267
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	TGGTTCTGTCAAACGGCATA	ATCGACAAAACCCGTACTCG	45.0	50.0	57.4	58.0	1711-1734 bp	1.0	75.7
ndhD_p2	TGGTTCTGTCAAACGGCATA	ATTCTGTTTTGAGCACGGGT	45.0	45.0	57.4	58.0	1671-1694 bp	1.0	75.6
ndhD_p3	TGGTTCTGTCAAACGGCATA	CGACAAAACCCGTACTCGAA	45.0	50.0	57.4	58.2	1709-1732 bp	1.0	73.9
ndhD_p4	TGGTTCTGTCAAACGGCATA	TCTGTTTTGAGCACGGGTTT	45.0	45.0	57.4	58.2	1669-1692 bp	1.0	73.8
ndhD_p5	TGGTTCTGTCAAACGGCATA	TTCTGTTTTGAGCACGGGTT	45.0	45.0	57.4	58.2	1670-1693 bp	1.0	73.8