Hotspot

rrn16-trnI-GAU

IRb · 105238-105532 · 295 bp

Back to Gymnosporia

Pi 0.0305

MarkerSeek score 70.00

Primer available yes

Label rrn16-trnI-GAU

Feature Metadata

label_name: rrn16-trnI-GAU
feature_type: igs
region: IRb
length_bp: 295
variable_sites: 9
pi: 0.0305
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rrn16-trnI-GAU_p1	CAAGGTAGCCGTACTGGAAG	CTATCCATGTGGCTGAGAGC	55.0	55.0	57.7	57.8	421 bp	1.0	78.0
rrn16-trnI-GAU_p2	CAAGGTAGCCGTACTGGAAG	GGCACAACGACGCAATTATC	55.0	50.0	57.7	58.2	389 bp	1.0	78.0
rrn16-trnI-GAU_p3	CAAGGTAGCCGTACTGGAAG	CGCTGATGAGCACATTGAAC	55.0	50.0	57.7	57.8	440 bp	1.0	77.9
rrn16-trnI-GAU_p4	AGTGACTGGAGTGAAGTCGT	CTATCCATGTGGCTGAGAGC	50.0	55.0	58.3	57.8	443 bp	1.0	77.8
rrn16-trnI-GAU_p5	AGTGACTGGAGTGAAGTCGT	CGCTGATGAGCACATTGAAC	50.0	50.0	58.3	57.8	462 bp	1.0	77.8