Hotspot

psaA-ycf3

LSC · 43227-43910 · 684 bp

Back to Gymnosporia

Pi 0.00293

MarkerSeek score 60.70

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 684
variable_sites: 2
pi: 0.00293
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	GCCCATTCCTCGAAAGAAGT	AAGCCGCCGAATATTGGAAA	50.0	45.0	57.9	58.2	857-859 bp	1.0	77.5
psaA-ycf3_p2	GCCCATTCCTCGAAAGAAGT	GCTATAGCGCTTACTCCTGG	50.0	55.0	57.9	57.6	834-836 bp	1.0	76.6
psaA-ycf3_p3	TGTTCTTGAGAAATGGCCGG	AAGCCGCCGAATATTGGAAA	50.0	45.0	58.5	58.2	882-884 bp	1.0	74.7
psaA-ycf3_p4	GCCCATTCCTCGAAAGAAGT	GATCAAGCCGCCGAATATTG	50.0	50.0	57.9	57.4	861-863 bp	1.0	74.6
psaA-ycf3_p5	GCCCATTCCTCGAAAGAAGT	GGAGGCTTGGTTCGATCAAG	50.0	55.0	57.9	58.6	874-876 bp	1.0	74.4