Hotspot

petN-psbM

LSC · 28901-29747 · 847 bp

Back to Gymnosporia

Pi 0.00236

MarkerSeek score 60.10

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 847
variable_sites: 2
pi: 0.00236
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	TAGTAAGTCTCGCTTGGGCT	CGATCAGTCAAAGTCAGGGC	50.0	55.0	58.2	58.6	965 bp	1.0	73.9
petN-psbM_p2	TAGTAAGTCTCGCTTGGGCT	GATCAGTCAAAGTCAGGGCG	50.0	55.0	58.2	58.6	964 bp	1.0	73.9
petN-psbM_p3	TAGTAAGTCTCGCTTGGGCT	CAGTCAAAGTCAGGGCGATA	50.0	50.0	58.2	57.3	961 bp	1.0	73.6
petN-psbM_p4	TAGTAAGTCTCGCTTGGGCT	ACGATCAGTCAAAGTCAGGG	50.0	50.0	58.2	57.2	966 bp	1.0	72.9
petN-psbM_p5	ATGGGGAAGAAGTGGACTCT	GATCAGTCAAAGTCAGGGCG	50.0	55.0	57.6	58.6	903 bp	1.0	72.3