Hotspot

atpH-atpI

LSC · 13277-14542 · 1266 bp

Back to Gymnosporia

Pi 0.00237

MarkerSeek score 60.10

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1266
variable_sites: 3
pi: 0.00237
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	AACAGAAGCGGCAGAAATCA	TTTTTGCAACTTTAGCCGCG	45.0	45.0	58.1	58.5	1352 bp	1.0	75.2
atpH-atpI_p2	CAGCAGCAATAACAGAAGCG	TTTTTGCAACTTTAGCCGCG	50.0	45.0	57.8	58.5	1362 bp	1.0	74.7
atpH-atpI_p3	AACAGAAGCGGCAGAAATCA	TAGGTGAATCCATGGAGGGT	45.0	50.0	58.1	57.4	1325 bp	1.0	74.7
atpH-atpI_p4	CAGCAGCAATAACAGAAGCG	TAGGTGAATCCATGGAGGGT	50.0	50.0	57.8	57.4	1335 bp	1.0	74.1
atpH-atpI_p5	AATAGAAGCAAGACCGACGG	TTTTTGCAACTTTAGCCGCG	50.0	45.0	57.7	58.5	1388 bp	1.0	73.7