Hotspot

trnS-GCU-trnG-UCC

LSC · 7539-8378 · 840 bp

Back to Gymnosporia

Pi 0.00239

MarkerSeek score 53.40

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 840
variable_sites: 2
pi: 0.00239
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	CAATCCGACGCTTTAGTCCA	CCCAGGGACCCCTTAACTAT	50.0	55.0	58.0	57.8	945-946 bp	1.0	78.6
trnS-GCU-trnG-UCC_p2	AATCCGACGCTTTAGTCCAC	CCCAGGGACCCCTTAACTAT	50.0	55.0	58.0	57.8	944-945 bp	1.0	78.6
trnS-GCU-trnG-UCC_p3	ACGGAAAGAGAGGGATTCGA	CCCAGGGACCCCTTAACTAT	50.0	55.0	58.2	57.8	999-1000 bp	1.0	77.4
trnS-GCU-trnG-UCC_p4	AGCAATCCGACGCTTTAGTC	CCCAGGGACCCCTTAACTAT	50.0	55.0	58.4	57.8	947-948 bp	1.0	75.8
trnS-GCU-trnG-UCC_p5	CGACGCTTTAGTCCACTCAG	CCCAGGGACCCCTTAACTAT	55.0	55.0	58.4	57.8	940-941 bp	1.0	75.6