Hotspot

trnQ-UUG-psbK

LSC · 6119-6475 · 357 bp

Back to Gymnosporia

Pi 0.0056

MarkerSeek score 44.90

Primer available yes

Label trnQ-UUG-psbK

Feature Metadata

label_name: trnQ-UUG-psbK
feature_type: igs
region: LSC
length_bp: 357
variable_sites: 2
pi: 0.0056
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 44.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnQ-UUG-psbK_p1	GACCAAAACCCGTTGCCTTA	ACAGATCCAGATCAAACTAAAGA	50.0	34.8	58.7	55.1	428 bp	1.0	40.4
trnQ-UUG-psbK_p2	GACCAAAACCCGTTGCCTTA	AAACAGATCCAGATCAAACT	50.0	35.0	58.7	52.0	430 bp	1.0	40.4
trnQ-UUG-psbK_p3	GACCAAAACCCGTTGCCTTA	AAAACAGATCCAGATCAAACT	50.0	33.3	58.7	53.0	431 bp	1.0	40.4
trnQ-UUG-psbK_p4	GAATAGCGGGACCAAAACCC	ACAGATCCAGATCAAACTAAAGA	55.0	34.8	58.9	55.1	437 bp	1.0	40.4
trnQ-UUG-psbK_p5	GAATAGCGGGACCAAAACCC	AAACAGATCCAGATCAAACT	55.0	35.0	58.9	52.0	439 bp	1.0	40.4