Hotspot

trnL-UAG-ccsA

Genome · 113904-114142 · 239 bp

Back to Gymnosphaera

Pi 0.0286

MarkerSeek score 59.80

Primer available yes

Label trnL-UAG-ccsA

Feature Metadata

label_name: trnL-UAG-ccsA
feature_type: igs
region: Genome
length_bp: 239
variable_sites: 12
pi: 0.0286
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAG-ccsA_p1	GTGAAATCGGTAGACACGCT	AATGTGAGCGAATATGTGCT	50.0	40.0	58.0	55.5	345-347 bp	1.0	63.1
trnL-UAG-ccsA_p2	TGAAATCGGTAGACACGCTG	AATGTGAGCGAATATGTGCT	50.0	40.0	58.0	55.5	344-346 bp	1.0	63.1
trnL-UAG-ccsA_p3	TAGACACGCTGCTCTTAGGA	AATGTGAGCGAATATGTGCT	50.0	40.0	57.9	55.5	335-337 bp	1.0	62.3
trnL-UAG-ccsA_p4	GGTAGACACGCTGCTCTTAG	AATGTGAGCGAATATGTGCT	55.0	40.0	57.8	55.5	337-339 bp	1.0	61.7
trnL-UAG-ccsA_p5	GTAGACACGCTGCTCTTAGG	AATGTGAGCGAATATGTGCT	55.0	40.0	57.8	55.5	336-338 bp	1.0	61.7