Hotspot

rps7-psbA

Genome · 99656-100526 · 871 bp

Back to Gymnosphaera

Pi 0.00344

MarkerSeek score 61.20

Primer available yes

Label rps7-psbA

Feature Metadata

label_name: rps7-psbA
feature_type: igs
region: Genome
length_bp: 871
variable_sites: 6
pi: 0.00344
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps7-psbA_p1	TGAATCAATGGATGCTGCCA	CGCGTCTTTCTAAAGTTGCG	45.0	50.0	57.9	58.1	999-1016 bp	1.0	78.1
rps7-psbA_p2	TGAATCAATGGATGCTGCCA	TAGACGGTTTTCAGTGCTGG	45.0	50.0	57.9	57.8	1058-1075 bp	1.0	78.0
rps7-psbA_p3	TCAATGGATGCTGCCAGAAA	CGCGTCTTTCTAAAGTTGCG	45.0	50.0	57.8	58.1	995-1012 bp	1.0	77.6
rps7-psbA_p4	TGAATCAATGGATGCTGCCA	GCGTCTTTCTAAAGTTGCGG	45.0	50.0	57.9	57.8	998-1015 bp	1.0	77.5
rps7-psbA_p5	TCAATGGATGCTGCCAGAAA	TAGACGGTTTTCAGTGCTGG	45.0	50.0	57.8	57.8	1054-1071 bp	1.0	77.4