Hotspot

trnP-UGG-psaJ

Genome · 65956-66213 · 258 bp

Back to Gymnosphaera

Pi 0.0304

MarkerSeek score 67.10

Primer available yes

Label trnP-UGG-psaJ

Feature Metadata

label_name: trnP-UGG-psaJ
feature_type: igs
region: Genome
length_bp: 258
variable_sites: 14
pi: 0.0304
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnP-UGG-psaJ_p1	AACAAACACGCTACCGAACT	AGGGGCCGTGGAAAAATAAG	45.0	50.0	58.1	58.2	327-332 bp	1.0	81.7
trnP-UGG-psaJ_p2	CCCAAAACAAACACGCTACC	AGGGGCCGTGGAAAAATAAG	50.0	50.0	57.9	58.2	332-337 bp	1.0	81.1
trnP-UGG-psaJ_p3	CCGATTCTTGGTGCTTGGTA	AGGGGCCGTGGAAAAATAAG	50.0	50.0	57.9	58.2	401-406 bp	1.0	80.7
trnP-UGG-psaJ_p4	CTTGGTGCTTGGTACATGGA	AGGGGCCGTGGAAAAATAAG	50.0	50.0	57.8	58.2	395-400 bp	1.0	80.0
trnP-UGG-psaJ_p5	TCTTGGTGCTTGGTACATGG	AGGGGCCGTGGAAAAATAAG	50.0	50.0	57.8	58.2	396-401 bp	1.0	80.0