Hotspot

ycf3-trnS-GGA

Genome · 45554-46033 · 480 bp

Back to Gymnosphaera

Pi 0.0219

MarkerSeek score 51.40

Primer available yes

Label ycf3-trnS-GGA

Feature Metadata

label_name: ycf3-trnS-GGA
feature_type: igs
region: Genome
length_bp: 480
variable_sites: 18
pi: 0.0219
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-trnS-GGA_p1	GTCATTTCTCTGAGATCTAGGCA	TGCTACACCTTAAACCGCTC	43.5	50.0	57.5	57.9	533-536 bp	1.0	54.5
ycf3-trnS-GGA_p2	TGTCATTTCTCTGAGATCTAGGC	TGCTACACCTTAAACCGCTC	43.5	50.0	57.5	57.9	534-537 bp	1.0	54.5
ycf3-trnS-GGA_p3	GTCATTTCTCTGAGATCTAGGCA	GTGCTACACCTTAAACCGCT	43.5	50.0	57.5	58.2	534-537 bp	1.0	53.6
ycf3-trnS-GGA_p4	TGTCATTTCTCTGAGATCTAGGC	GTGCTACACCTTAAACCGCT	43.5	50.0	57.5	58.2	535-538 bp	1.0	53.6
ycf3-trnS-GGA_p5	GTGGTCGGTAGAATTTGTAACA	TGCTACACCTTAAACCGCTC	40.9	50.0	56.4	57.9	595-598 bp	1.0	53.5