Hotspot

psbD-trnT-GGU

Genome · 36114-37710 · 1597 bp

Back to Gymnosphaera

Pi 0.0222

MarkerSeek score 61.50

Primer available yes

Label psbD-trnT-GGU

Feature Metadata

label_name: psbD-trnT-GGU
feature_type: igs
region: Genome
length_bp: 1597
variable_sites: 63
pi: 0.0222
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbD-trnT-GGU_p1	TTTTCCAATGGCGATGGTCA	TGGTAGGCCCCTTTAACTCA	45.0	50.0	58.1	58.0	1696-1701 bp	1.0	82.1
psbD-trnT-GGU_p2	TTTCCAATGGCGATGGTCAT	TGGTAGGCCCCTTTAACTCA	45.0	50.0	57.9	58.0	1695-1700 bp	1.0	81.6
psbD-trnT-GGU_p3	CGGACCAACCTACGAAAACA	TGGTAGGCCCCTTTAACTCA	50.0	50.0	58.1	58.0	1778-1783 bp	1.0	81.5
psbD-trnT-GGU_p4	ATCTTTCGGCTCCTTGGAAG	TGGTAGGCCCCTTTAACTCA	50.0	50.0	57.6	58.0	1717-1722 bp	1.0	79.4
psbD-trnT-GGU_p5	CTTTCGGCTCCTTGGAAGAT	TGGTAGGCCCCTTTAACTCA	50.0	50.0	57.6	58.0	1715-1720 bp	1.0	79.4