Hotspot

trnC-GCA-trnG-GCC

Genome · 30599-31894 · 1296 bp

Back to Gymnosphaera

Pi 0.0112

MarkerSeek score 60.30

Primer available yes

Label trnC-GCA-trnG-GCC

Feature Metadata

label_name: trnC-GCA-trnG-GCC
feature_type: igs
region: Genome
length_bp: 1296
variable_sites: 27
pi: 0.0112
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 60.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-trnG-GCC_p1	ATGTTGTATCAGGCGACACC	GTTCGATTCCCGCTATCCG	50.0	57.9	58.0	58.2	1381-1399 bp	1.0	73.8
trnC-GCA-trnG-GCC_p2	GGATTTGCAATCCTCCGTCT	GTTCGATTCCCGCTATCCG	50.0	57.9	58.0	58.2	1339-1357 bp	1.0	73.8
trnC-GCA-trnG-GCC_p3	CAGATTCGAACTGGGGGATG	GTTCGATTCCCGCTATCCG	55.0	57.9	58.0	58.2	1361-1379 bp	1.0	73.7
trnC-GCA-trnG-GCC_p4	AGGATTTGCAATCCTCCGTC	GTTCGATTCCCGCTATCCG	50.0	57.9	58.0	58.2	1340-1358 bp	1.0	73.7
trnC-GCA-trnG-GCC_p5	ATGTTGTATCAGGCGACACC	GGTTCGATTCCCGCTATCC	50.0	57.9	58.0	57.8	1382-1400 bp	1.0	73.6