Hotspot

ndhF

SSC · 112581-114731 · 2151 bp

Back to Gymnospermium

Pi 0.00731

MarkerSeek score 44.10

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2151
variable_sites: 47
pi: 0.00731
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 44.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	ACGAAGGGGACTAAACAGGA	AGAACTGGTGAATCGGAAACA	50.0	42.9	58.0	57.5	2640-2782 bp	0.875	68.8
ndhF_p2	CCGGGTGAATGGAAAGGAAA	AGAACTGGTGAATCGGAAACA	50.0	42.9	58.1	57.5	2530-2672 bp	0.875	68.5
ndhF_p3	CCTTCCCCTTCCCTTTGTTC	AGAACTGGTGAATCGGAAACA	55.0	42.9	58.1	57.5	2595-2737 bp	0.875	68.4
ndhF_p4	TTTAGGCAGAATACCGTCGC	AGAACTGGTGAATCGGAAACA	50.0	42.9	58.1	57.5	2755-2897 bp	0.875	68.2
ndhF_p5	AGAGATCCGGGTGAATGGAA	AGAACTGGTGAATCGGAAACA	50.0	42.9	58.1	57.5	2536-2678 bp	0.875	68.2