Hotspot

rpl33-rps18

LSC · 69317-69631 · 315 bp

Back to Gymnospermium

Pi 0.101

MarkerSeek score 74.00

Primer available yes

Label rpl33-rps18

Feature Metadata

label_name: rpl33-rps18
feature_type: igs
region: LSC
length_bp: 315
variable_sites: 71
pi: 0.101
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 74.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl33-rps18_p1	ACGATTCATGGGGAGATAAAGA	TGGATTTAAGAAAAAGCTTGGA	40.9	31.8	56.7	54.2	320-425 bp	1.0	52.0
rpl33-rps18_p2	ACGATTCATGGGGAGATAAAGA	TTGGATTTAAGAAAAAGCTTGGA	40.9	30.4	56.7	55.0	321-426 bp	1.0	52.0
rpl33-rps18_p3	CATACGATTCATGGGGAGAT	TGGATTTAAGAAAAAGCTTGGA	45.0	31.8	54.4	54.2	323-428 bp	1.0	52.0
rpl33-rps18_p4	ACGATTCATGGGGAGATAAAGAA	TGGATTTAAGAAAAAGCTTGGA	39.1	31.8	57.4	54.2	320-425 bp	1.0	52.0
rpl33-rps18_p5	ACGATTCATGGGGAGATAAAGA	TGGATTTAAGAAAAAGCTTGGAT	40.9	30.4	56.7	54.7	320-425 bp	1.0	52.0