Hotspot

psbC-trnS-UGA

LSC · 36664-36835 · 172 bp

Back to Gymnospermium

Pi 0.0385
MarkerSeek score 80.40
Primer available yes
Label psbC-trnS-UGA

Feature Metadata

label_name
psbC-trnS-UGA
feature_type
igs
region
LSC
length_bp
172
variable_sites
15
pi
0.0385
unique_haplotype_count
8
primer_available
yes
alignment_reliability
1.00
markerseek_score
80.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbC-trnS-UGA_p1 AGCTGCAGCAGGATTTGAAA GGTTGATAGCTCCGGTCTTG 45.0 55.0 58.4 58.1 319-326 bp 1.0 82.7
psbC-trnS-UGA_p2 GCTGCAGCAGGATTTGAAAA GGTTGATAGCTCCGGTCTTG 45.0 55.0 57.6 58.1 318-325 bp 1.0 82.2
psbC-trnS-UGA_p3 AGCTGCAGCAGGATTTGAAA GATGGCCGAGTGGTTGATAG 45.0 55.0 58.4 58.1 330-337 bp 1.0 82.0
psbC-trnS-UGA_p4 GCTGCAGCAGGATTTGAAAA GATGGCCGAGTGGTTGATAG 45.0 55.0 57.6 58.1 329-336 bp 1.0 81.4
psbC-trnS-UGA_p5 TATTCGTGGGCCATTTGTGG GGTTGATAGCTCCGGTCTTG 50.0 55.0 58.5 58.1 359-366 bp 1.0 80.8