Hotspot

trnR-UCU-atpA

LSC · 10170-10293 · 124 bp

Back to Gymnospermium

Pi 0.0383

MarkerSeek score 65.90

Primer available yes

Label trnR-UCU-atpA

Feature Metadata

label_name: trnR-UCU-atpA
feature_type: igs
region: LSC
length_bp: 124
variable_sites: 16
pi: 0.0383
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-atpA_p1	GGTTCAAATCCTATTGGACGC	ACCGAAGAGGCAGAAGTACT	47.6	50.0	57.3	58.1	224-226 bp	1.0	71.6
trnR-UCU-atpA_p2	GGTTCAAATCCTATTGGACGC	TGACCGAAGAGGCAGAAGTA	47.6	50.0	57.3	58.1	226-228 bp	1.0	71.5
trnR-UCU-atpA_p3	GGTTCAAATCCTATTGGACGC	CATTGACCGAAGAGGCAGAA	47.6	50.0	57.3	57.9	229-231 bp	1.0	71.2
trnR-UCU-atpA_p4	GGTTCAAATCCTATTGGACGC	AAGACATTGACCGAAGAGGC	47.6	50.0	57.3	57.9	233-235 bp	1.0	71.1
trnR-UCU-atpA_p5	GGTTCAAATCCTATTGGACGC	GACCGAAGAGGCAGAAGTAC	47.6	55.0	57.3	57.7	225-227 bp	1.0	69.9