Hotspot

psbI-trnS-GCU

LSC · 8187-8333 · 147 bp

Back to Gymnospermium

Pi 0.0256

MarkerSeek score 62.90

Primer available yes

Label psbI-trnS-GCU

Feature Metadata

label_name: psbI-trnS-GCU
feature_type: igs
region: LSC
length_bp: 147
variable_sites: 13
pi: 0.0256
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbI-trnS-GCU_p1	ATGATCCAGGGCGTAATCCT	TAAAGCGGCGGATTGCTAAT	50.0	45.0	58.3	58.0	249-275 bp	1.0	82.9
psbI-trnS-GCU_p2	ATGATCCAGGGCGTAATCCT	TTTTTCGTACCGAGGGTTCG	50.0	50.0	58.3	58.2	217-243 bp	1.0	82.2
psbI-trnS-GCU_p3	ATGATCCAGGGCGTAATCCT	ATTGGGAGAGATGGCTGAGT	50.0	50.0	58.3	58.1	273-299 bp	1.0	81.9
psbI-trnS-GCU_p4	AATGATCCAGGGCGTAATCC	TAAAGCGGCGGATTGCTAAT	50.0	45.0	57.4	58.0	250-276 bp	1.0	80.4
psbI-trnS-GCU_p5	ATGATCCAGGGCGTAATCCT	ATGGCTGAGTGGTCTAAAGC	50.0	50.0	58.3	57.6	263-289 bp	1.0	79.6