Hotspot

ycf1

SSC · 117805-123288 · 5484 bp

Back to Gymnocladus

Pi 0.39

MarkerSeek score 75.90

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5484
variable_sites: 1911
pi: 0.39
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.89
markerseek_score: 75.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TGAACTGCCGTGATTGCTTA	CGAAACGGCTGCCATTATTG	45.0	50.0	57.8	58.2	684 bp	0.5	57.2
ycf1_p2	TGAACTGCCGTGATTGCTTA	GAAACGGCTGCCATTATTGG	45.0	50.0	57.8	57.8	683 bp	0.5	56.9
ycf1_p3	AATTGAACTGCCGTGATTGC	CGAAACGGCTGCCATTATTG	45.0	50.0	57.6	58.2	687 bp	0.5	55.8
ycf1_p4	AATTGAACTGCCGTGATTGC	GAAACGGCTGCCATTATTGG	45.0	50.0	57.6	57.8	686 bp	0.5	55.5
ycf1_p5	TGAACTGCCGTGATTGCTTA	AACGGCTGCCATTATTGGTA	45.0	45.0	57.8	57.6	681 bp	0.5	55.2