Hotspot

ndhH

SSC · 124037-125218 · 1182 bp

Back to Gymnocladus

Pi 0.458

MarkerSeek score 76.20

Primer available yes

Label ndhH

Feature Metadata

label_name: ndhH
feature_type: gene
region: SSC
length_bp: 1182
variable_sites: 372
pi: 0.458
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.69
markerseek_score: 76.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhH_p1	GAAAACCCCGGATCGATTGA	TCAGGTCCAATACGCTGTTG	50.0	50.0	58.0	57.9	1705 bp	0.5	59.0
ndhH_p2	AGAAAACCCCGGATCGATTG	TCAGGTCCAATACGCTGTTG	50.0	50.0	58.0	57.9	1706 bp	0.5	58.9
ndhH_p3	GAAAACCCCGGATCGATTGA	CGAACAGAAAGGAGCATGGA	50.0	50.0	58.0	57.9	2110 bp	0.5	58.9
ndhH_p4	AGAAAACCCCGGATCGATTG	CGAACAGAAAGGAGCATGGA	50.0	50.0	58.0	57.9	2111 bp	0.5	58.9
ndhH_p5	GCACACACGCATTGTCTTAC	TCAGGTCCAATACGCTGTTG	50.0	50.0	58.0	57.9	2071 bp	0.5	58.9