Hotspot

ndhG

SSC · 129176-129706 · 531 bp

Back to Gymnocladus

Pi 0.456

MarkerSeek score 79.80

Primer available yes

Label ndhG

Feature Metadata

label_name: ndhG
feature_type: gene
region: SSC
length_bp: 531
variable_sites: 209
pi: 0.456
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.86
markerseek_score: 79.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG_p1	CCTGGTCGTATCAATAAGGTCA	TTTCGGCTCGTGATCAATCC	45.5	50.0	57.3	58.3	915 bp	0.5	35.9
ndhG_p2	CCTGGTCGTATCAATAAGGTCA	CTGGTCCAATAGCTGCTTCA	45.5	50.0	57.3	57.6	1073 bp	0.5	35.4
ndhG_p3	CCTGGTCGTATCAATAAGGTCA	TGGTCCAATAGCTGCTTCAG	45.5	50.0	57.3	57.6	1072 bp	0.5	35.4
ndhG_p4	CCTGGTCGTATCAATAAGGTCA	CGGCTCGTGATCAATCCATA	45.5	50.0	57.3	57.6	912 bp	0.5	35.2
ndhG_p5	CCTGGTCGTATCAATAAGGTCA	TGTTCGAGCATCATTGACCA	45.5	45.0	57.3	57.5	852 bp	0.5	34.9