Hotspot

ndhF

SSC · 136069-138306 · 2238 bp

Back to Gymnocladus

Pi 0.462

MarkerSeek score 79.30

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2238
variable_sites: 881
pi: 0.462
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.85
markerseek_score: 79.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	TTTTTCTTGTAGCCCGGCTT	TGTGGATGTGTCTTCTTCCG	45.0	50.0	58.0	57.5	724 bp	0.5	56.3
ndhF_p2	CACCCTTTGCCTGTTTTTGG	TGTGGATGTGTCTTCTTCCG	50.0	50.0	58.0	57.5	319 bp	0.5	56.0
ndhF_p3	CCACCCTTTGCCTGTTTTTG	TGTGGATGTGTCTTCTTCCG	50.0	50.0	58.0	57.5	320 bp	0.5	56.0
ndhF_p4	ATTTGGCGGTTCAGTTGCTA	TGTGGATGTGTCTTCTTCCG	45.0	50.0	58.1	57.5	846 bp	0.5	55.6
ndhF_p5	TGGCGGTTCAGTTGCTAAAT	TGTGGATGTGTCTTCTTCCG	45.0	50.0	58.1	57.5	843 bp	0.5	55.6