Hotspot

ndhA

SSC · 125220-127699 · 2480 bp

Back to Gymnocladus

Pi 0.449

MarkerSeek score 73.30

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2480
variable_sites: 696
pi: 0.449
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.62
markerseek_score: 73.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	AGAGTTGAAGCACCAAAGGG	TGCAGCTCGTACTGTTTGTT	50.0	45.0	58.0	58.1	2815 bp	0.5	59.5
ndhA_p2	AGAGTTGAAGCACCAAAGGG	TTGCAGCTCGTACTGTTTGT	50.0	45.0	58.0	58.1	2816 bp	0.5	59.5
ndhA_p3	AGTTGAAGCACCAAAGGGAG	TGCAGCTCGTACTGTTTGTT	50.0	45.0	58.0	58.1	2813 bp	0.5	59.5
ndhA_p4	AGTTGAAGCACCAAAGGGAG	TTGCAGCTCGTACTGTTTGT	50.0	45.0	58.0	58.1	2814 bp	0.5	59.5
ndhA_p5	GTTGAAGCACCAAAGGGAGA	TGCAGCTCGTACTGTTTGTT	50.0	45.0	58.0	58.1	2812 bp	0.5	59.4