Hotspot

ccsA

SSC · 132677-133648 · 972 bp

Back to Gymnocladus

Pi 0.462

MarkerSeek score 75.20

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 972
variable_sites: 293
pi: 0.462
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.65
markerseek_score: 75.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	GGTGTTTGTGAGAACCCCTT	GTGAAATCGGTAGACACGCT	50.0	50.0	57.9	58.0	1300 bp	0.5	59.4
ccsA_p2	GGTGTTTGTGAGAACCCCTT	TGAAATCGGTAGACACGCTG	50.0	50.0	57.9	58.0	1299 bp	0.5	59.4
ccsA_p3	CGGTTTTGTTGCGGAATTGA	GTGAAATCGGTAGACACGCT	45.0	50.0	57.9	58.0	1742 bp	0.5	58.9
ccsA_p4	CGGTTTTGTTGCGGAATTGA	TGAAATCGGTAGACACGCTG	45.0	50.0	57.9	58.0	1741 bp	0.5	58.9
ccsA_p5	CAGTTGACAAGGTCGAAGCT	GTGAAATCGGTAGACACGCT	50.0	50.0	58.1	58.0	1461 bp	0.5	58.9