Hotspot

rpl32-ndhF

SSC · 135054-136068 · 1015 bp

Back to Gymnocladus

Pi 0.43

MarkerSeek score 80.90

Primer available yes

Label rpl32-ndhF

Feature Metadata

label_name: rpl32-ndhF
feature_type: igs
region: SSC
length_bp: 1015
variable_sites: 373
pi: 0.43
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.86
markerseek_score: 80.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-ndhF_p1	TCAATGCTGCCCAATATCCC	TAGGGCGTGTGAACCAAAAA	50.0	45.0	58.0	57.9	1611 bp	0.5	59.6
rpl32-ndhF_p2	CAATGCTGCCCAATATCCCT	TAGGGCGTGTGAACCAAAAA	50.0	45.0	58.0	57.9	1610 bp	0.5	59.5
rpl32-ndhF_p3	TCAATGCTGCCCAATATCCC	AGCAACTGAACCGCCAAATA	50.0	45.0	58.0	58.1	1820 bp	0.5	59.2
rpl32-ndhF_p4	TCAATGCTGCCCAATATCCC	ATTTAGCAACTGAACCGCCA	50.0	45.0	58.0	58.1	1824 bp	0.5	59.2
rpl32-ndhF_p5	TCAATGCTGCCCAATATCCC	TAGCAACTGAACCGCCAAAT	50.0	45.0	58.0	58.1	1821 bp	0.5	59.2