Hotspot

ndhD-ccsA

SSC · 132401-132676 · 276 bp

Back to Gymnocladus

Pi 0.544

MarkerSeek score 66.80

Primer available yes

Label ndhD-ccsA

Feature Metadata

label_name: ndhD-ccsA
feature_type: igs
region: SSC
length_bp: 276
variable_sites: 81
pi: 0.544
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.54
markerseek_score: 66.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD-ccsA_p1	CGGTTTTGTTGCGGAATTGA	GGGCTACGCAGCTCTTTTAT	45.0	50.0	57.9	58.0	1130 bp	0.5	58.6
ndhD-ccsA_p2	CAGTTGACAAGGTCGAAGCT	GGGCTACGCAGCTCTTTTAT	50.0	50.0	58.1	58.0	849 bp	0.5	58.6
ndhD-ccsA_p3	TCAGTTGACAAGGTCGAAGC	GGGCTACGCAGCTCTTTTAT	50.0	50.0	58.1	58.0	850 bp	0.5	58.6
ndhD-ccsA_p4	TTATCTCGACGAAATGGGCG	GGGCTACGCAGCTCTTTTAT	50.0	50.0	58.2	58.0	1229 bp	0.5	58.4
ndhD-ccsA_p5	GGGCCGCGAGAGTTATTTAT	GGGCTACGCAGCTCTTTTAT	50.0	50.0	57.8	58.0	934 bp	0.5	58.3