Hotspot

ndhG-ndhE

SSC · 129707-129954 · 248 bp

Back to Gymnocladus

Pi 0.435

MarkerSeek score 67.00

Primer available yes

Label ndhG-ndhE

Feature Metadata

label_name: ndhG-ndhE
feature_type: igs
region: SSC
length_bp: 248
variable_sites: 60
pi: 0.435
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.56
markerseek_score: 67.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG-ndhE_p1	CCATTTTGTAGCTGCAGCAC	ACATCCGTCCCAAGGTATCA	50.0	50.0	58.0	58.1	1296 bp	0.5	58.9
ndhG-ndhE_p2	TTCGTTGGGACTGGTTCTTG	ACATCCGTCCCAAGGTATCA	50.0	50.0	58.0	58.1	1350 bp	0.5	58.7
ndhG-ndhE_p3	TTTCTGCCTTTTCGTTGGGA	ACATCCGTCCCAAGGTATCA	45.0	50.0	57.9	58.1	1360 bp	0.5	58.5
ndhG-ndhE_p4	CCATTTTGTAGCTGCAGCAC	GTTCTCGGAGCAGAAGCAAT	50.0	50.0	58.0	58.3	1328 bp	0.5	57.7
ndhG-ndhE_p5	CCATTTTGTAGCTGCAGCAC	CTCTGTTCTCGGAGCAGAAG	50.0	55.0	58.0	57.7	1332 bp	0.5	57.7